Deformed Wing Virus is an RNA virus that infects the honeybee and is distantly related to the human poliovirus. We are interested in studying this virus because it may be involved in the recent loss of honeybees and may be able to affect the behavior of the honeybees. We are studying the proteins that this virus makes by fusing them with the green fluorescent protein. This allows us to determine where in the cell the proteins go and may help us identify their function during the virus life cycle. These initial experiments were done in human cells, but we plan to repeat these experiments in various types of insect cells.
These panels show a mammalian cell expressing different portions of the DWV nonstructural proteins fused to the green fluorescent protein. The green shows the location of the viral protein while the blue is a DNA stain that shows the location of the nucleus. Most of these protein (panels A and B) localize to the cytoplasm of the cell, which is where DWV replicates, and may be concentrated in specific areas within the cytoplasm. Interestingly, the 3CD protein (panel C), appears to localize to the nucleus of the cell. A similar protein from poliovirus also localizes to the nucleus and this may represent a conservation of protein function between these two viruses. Images taken by Cierra Danko '10.
This material is based upon work supported by the National Science Foundation under Grant No. 0923010. Any opinions, findings and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the National Science Foundation (NSF).
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