Microscope, PCM2000 scanner module, PCM2000 controller, computer display monitors (2 units), air-cooled argon-ion laser, HeNe laser, z-axis drive motor and contoller.
Nikon Eclipse E800
Air-cooled argon-ion-laser system. Spectra-Physics laser head 163-C02 laser power supply 263-C00, and remote controller 361C
HeNe laser system (543.5 nm) Uniphase laser head 1676
HeNe laser system (633 nm). Uniphase laser head 1135
488 nm excitation + one-channel detection: FITC single-color staining
488 nm excitation + two-channel detection: FITC + PI two-color staining
543 nm excitation + one-channel detection: Rhodamine single-color staining, Texas red single color staining
633 nm excitation + one-channel detection: CY5 single-color staining
488 nm + 543 nm simultaneous excitation + two-color channel detection: FITC + Rhodamine two-color staining
488nm + 633 simultaneous excitation + two-channel detection: FITC + CY5 two-color staining
Z-axis drive motor:
Minimum increment for movement: 0.1 micron
Galvano-mirror scanner with control (x-axis, y-xis).
ImageSize (at the primary image plane):
A rectangle inscribed in a circle with a diameter of 18 mm.
Adjustable between 1x and 10x changeable every 0.1x.
1280x1024, 640x512, 320x256.
Image Acquisition Mode:
X-Y scanning, X-t scanning, fast scanning (2x – 4x), slow scanning (1/2, ¼, 1/8, 1/16, 1/32), Preview mode, Integration function (between 1 and 255 changeable every one step).
Two photo multiplier tubes are mounted.
Protection against Excessive light:
If the light incident on channels 1 and/or 2 exceeds the specified limit, the alarm indicator lights on, and image acquisition is interrupted for PMT protection.
Approximately 2500 W.
Display During Image Acquisition:
Individual display for each channel: selectable from red, green, blue, red-green mixture, red-blue mixture,green-blue mixture, and monochrome. Simultaneous display of two channels: red (channel 1), green (channel 2).